Production of polyclonal antibodies in rabbits.
نویسندگان
چکیده
The generation of polyclonal antibodies to an antigen of interest is an important technique applicable to many areas of biological research. In this chapter, we describe a basic immunization procedure designed to generate polyclonal antisera in rabbits and two methods that are commonly employed in the subsequent preliminary characterization of antipeptide antibodies raised in this way.
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ورودعنوان ژورنال:
- Methods in molecular biology
دوره 295 شماره
صفحات -
تاریخ انتشار 2005